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HCV 3'
NTR
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The 3' Nontranslated Region
(3'NTR) is the most conserved region of the HCV genome Kolykhalov
et al., J. Virology 70:3363, 1996 ;
Blight, KJ and Rice, CM., J. Virology 71:7345, 1997).
It is likely to be a critical regulatory region and it has
been shown to be required for replication of an infectious
clone of HCV in chimpanzees Kolykhalov
et al., Science 277:570, 1997. The 3' NTR is, therefore,
an excellent genomic (RNA) target for an anti-HCV therapy. |
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Translation initiation Internal
ribosome entry site
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Minus-strand initiation
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HCV 3' NTR 98
bp terminus
1a GGTGGCTCCATCTTAGCCCTAGTCACGGCTAGCTGTGAAAGGTCCGTGAGCCGCATGACTGCAGAGAGTGCTGATACTGGCCTCTCTGCAGATCATGT
1a ..................................................................................................
1a ...................................................g..................................c...........
1a ...................................................g.....................................t........
1b ..........................................................................a.......................
1b ..................................................................................................
1b ..................................................................................c...............
1b ..................................................................................c...............
1b ................................................................g.................................
1b ..................................................................................................
1b ..................................................................................................
1b ..................................................................................................
1b ..................................................................................................
1b ..................................................................................................
1b ..................................................................................................
1b ..................................................................................................
2a .......................................................................c.ta.....t.................
2a .......................................................................c.ta.....t.................
2b .......................................................................c.ta.....t.................
3a ..................................................................................................
3b ......................................................t..................ta....................a..
3 ......................................................t........................................a..
4? ..................................................................................................
4a ..........................................................................a.......................
6 ..................................................................................................
7 ...........................................................................................t......
8 ..........................................................................a.......................
9 ........................................................................../.......................
Nucleotide
sequence in the 3' NTR of 28 independently cloned isolates of
HCV representing the genotypes shown on the left. Dots
represent the same nucleotides as in the first isolate.
U.S. 5,874,565 entitled (and related
EP 0856051)
was assigned to Washington
University and licensed exclusively to Apath.
Apath
has already sublicensed exclusive rights to the 3' NTR for
diagnostic applications to a major diagnostics company. Rights
to therapeutic applications are currently available for
exclusive
licensing to interested pharmaceutical companies.
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